Janus nanoparticles are attaining profound interest due to their exciting and multifunctional properties generated from their asymmetric structures. However, control over synthesis, especially in solutions with small sizes, is a challenging concern. Herein, a simple hydrothermal method was applied to integrate the two highly biocompatible materials to design a Fe₃O₄-TiO₂ Janus structure. The structural characterization techniques were justified the asymmetric nature of the prepared nanocomposites. The average size of the JNPs was ~30 ± 2 nm, whereas the hydrodynamic size of Pluronic® F-127-coated JNPs was ~120 nm. The PF-127 modified Fe₃O₄-TiO₂ JNPs showed good biocompatibility, cancer cell targeting, and reactive oxygen species (ROS) generation ability. The designed nanocomposites yielded excellent r₂ relaxivity 18.2 mM^-1s^-1 and MR-image enchantment, suggesting their capability as a T₂-weighted contrast agent. Moreover, the PF127@Fe₃O₄-TiO₂ JNCs probe caused efficient photo-toxicity efficiency in vitro on 4T1 breast cancer cells under low-intensity UV light without any adverse effects to the control group. The present research provides a simple synthesis method and possesses an excellent imaging-guided therapeutic platform for future cancer treatment and biomedical applications.